Sterility Testing of the sodium cromoglycate Eye Drop Formulation Essay

Sterility Testing of the sodium cromoglycate Eye Drop Formulation - Essay Example ingested into the human body, the area in and around the pharmacy should be kept free from any microorganisms that might spread infection within the human body. The specific eye drop formulation used in this lab consists of several different key components that increase its efficacy. In this case, the components in question are Benzylpenicillin and para-aminobenzoic acid. The effectiveness of these particular products in sterilising against the presence of microorganisms is seen once the products themselves are activated. As a result, in order for Benzylpenicillin to break down penicillin, it does need to first be inactivated by banzylpenicillinase. In addition, para-aminobenzoic acid produces folic acid that can hinder some of the processes taking place within any bacteria that is present, effectively sterilising them (Regamy 2004). In an effort to test for the presence of a wide range of microorganisms, a number of different specimen should be used. In this case, sulfonamid, phenyl mercuric nitrate, sodium metabisulphite, and sodium edentate were used as they provide an environment that allows for the ready testing of a variety of microorganisms, as opposed to a situation that would result if the eye drop formulation were composed of only one compound. Sterilisation is a process that involves the utilisation of chemical compounds that are designed to inactivate the functioning of disease causing microorganisms (Akers 2005). This experiment involved the use of several biological and physicochemical indicators that included quaternary ammonium compound (QAC), penicillin, sulphonamide, phenol sodium edentate (EDTA), and mercurials. The methods used to test for actual sterilisation included non-ionic detergent, beta lactase, the use of p-aminobenzoic and dilution method, and thioglycollate. The results revealed that the oxidised and reduced Brewer’s medium solutions retained their colours of green and hello respectively. This confirms the presence of